Yanmei He, Xiao Lin, Wanwan Zhong, Xingyan Liu, Xiuyan Yang, Youlian Yao, Meimei Li, Jianyong Zhang, The discovery of pharmacodynamic material basis and mechanism of Shuganning injection in treating damp-heat jaundice syndrome: Combining metabolomics and serum pharmacochemistry, Journal of Pharmaceutical and Biomedical Analysis, Volume 272, 2026, 117380, ISSN 0731-7085.

发表期刊：Journal of Pharmaceutical and Biomedical Analysis

发表时间：2026

Abstract:

Shuganning injection (SGNI) is derived from Yinchenhao decoction and has the functions of promoting diuresis, reducing jaundice and protecting the liver. It is primarily utilized in the treatment of liver diseases, particularly damp-heat jaundice. However, the pharmacodynamic material basis and specific mechanisms remain unclear. Therefore, this study aims to elucidate the pharmacodynamic material basis and mechanism of SGNI in the treatment of damp-heat jaundice syndrome (DHJS). High-throughput metabolomics was employed to identify biomarkers associated with DHJS. The migratory prototype components and metabolites present in the blood of SGNI were characterized through serum pharmacochemistry. Correlation analysis was conducted to associate the biomarkers of DHJS with the migrating components in the blood of SGNI, clarifying the pharmacodynamic basis of SGNI in treating DHJS. Furthermore, metabolic pathway enrichment analysis was performed to explore the endogenous core metabolites and key metabolic pathways, providing insights into the mechanism of SGNI in treating DHJS from the metabolic perspective. Finally, a total of 25 metabolites were identified as biomarkers for DHJS, along with 24 prototype migratory components and 38 migratory metabolites of SGNI. It was established that the pharmacodynamic substance basis of SGNI in the treatment of DHJS comprises 15 components. These components primarily exert their effects through key metabolic pathways, including pentose and glucuronate interconversions, arachidonic acid metabolism, and primary bile acid biosynthesis. In conclusion, SGNI demonstrates a significant therapeutic effect on DHJS. It has preliminarily elucidated the pharmacodynamic substance basis and mechanism of action at the metabolic level, offering a scientific foundation for the clinical application and further development of SGNI.

https://doi.org/10.1016/j.jpba.2026.117380